![]() ![]() You will be prompted to identify the key findings.Īs expected when working as a part of any research group, you will be required to report your findings back to the head of the pharmaceutical research and decipher whether your western blot results validate the hypothesis you decided at the beginning after learning more about the research project. Now that you have performed the western blot, you will be given the opportunity to learn how to interpret such results and detect the relevant information from it that you require to apply it to the case of breast treatment. In western blotting, protein samples are separated in a polyacrylamide gel by electrophoresis and transferred to a membrane. Quantification of protein extracts prior to Western blotting is crucial first step in generation of accurate data. Lastly, the computational molecular weight analysis of MYC-FLAG was 25.1 kD unlike the Western Blot varied from 30, 37, 51, 120. Understanding of the physiology and cellular location of the target protein will allow for optimal protein extraction outcomes from various sample source. The GST computational analysis molecular weight was 47.5 kD whereas the Western Blot was 32, 52, and 55 meaning the Western Blot was slightly higher. To investigate this idea, you will perform a western blot experiment to test for the expression of vascular endothelial growth factor receptor (VEGFR) expression in patient samples compared to healthy tissue samples. Quantitative approach to Western Blotting. You will mainly apply the method of Western Blotting and SDS-PAGE to the case study of finding an effective treatment for breast cancer.Īnalyze patient samples by western blottingĪs a researcher in the R&D department of a big pharma company, your mission will be to test the hypothesis that increased blood vessel growth, also called angiogenesis, plays a role in breast cancer development. Other types of graphs, such as line graphs, were much more common in cell biology papers (11) than neuroscience papers (2). What is the western blot and what can it be used for? In this simulation, you will learn how tumor cells send signals to surrounding cells to help promote tumor growth, and how this signal is transmitted inside the cell. Among these papers, bar graphs were the most common type of graph used to present western blot data (44 neurosciences 30 cell biology), followed by dot plots (40 neurosciences 22 cell biology). This short, targeted simulation is adapted from the full-length “Signal Transduction” simulation.
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